Serbia's decision to omit Novak Djokovic from the doubles was "like leaving a Ferrari in the garage", said Radek Stepanek.
The Czech Republic won their second successive Davis Cup title after Radek Stepanek beat Dusan Lajovic 6-3 6-1 6-1.
Novak Djokovic won the opening match of the Davis Cup for Serbia before Tomas Berdych restored parity for the Czech Republic.
The International Tennis Federation (ITF) is confident its anti-doping programmes are working effectively.
Serbia's hopes of winning a second Davis Cup title have suffered a blow with second-ranked Janko Tipsarevic pulling out.
Winning a second Davis Cup title with Serbia would cap a fantastic end to the season for Novak Djokovic.
Andy Murray has called Victor Troicki and Marin Cilic "unprofessional", adding to the debate about tennis' anti-doping rules.
Novak Djokovic says he has lost all trust in the anti-doping programme in tennis and fears he could become a victim of its "negligence".
Viktor Troicki said his dream of being a "top player" had been taken away after his doping ban was reduced to 12 months from 18.
Serbia's Janko Tipsarevic faces a race against time to get fit for next month's Davis Cup final against the Czech Republic after being hampered by a foot injury
Serbia's Viktor Troicki has appealed to the CAS over his 18-month suspension for violating doping regulations.
Banned Serbian tennis player Viktor Troicki is "innocent" of a doping violation, world number one Novak Djokovic has said.
Round-up: Florian Mayer defied his journeyman status to knock Bernard Tomic out in the first round of the Montreal Masters.
Viktor Troicki will appeal to the Court of Arbitration (CAS) against the 18-month ban slapped on him.
Serbian tennis star Viktor Troicki has been banned for 18 months for refusing to give a blood sample at a doping control.
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