"The kit man Denilson Macedo ran onto the pitch from the tunnel entrance and attempted to attack (the linesman). He had to be restrained by police," Brazil's disciplinary tribunal said in a statement, adding that Macedo also swore at and insulted the official.
Team manager Rodrigo Henriques also faces a ban after Fluminense officials refused to give Macedo's name to the referee or police, saying he would be punished internally.
Fluminense were furious after having a goal disallowed late in the 0-0 draw against Brazilian championship leaders Atletico Mineiro. The hearing will take place on next Monday.
Brazilian domestic football, where even ball boys can be a source of controversy, is plagued by brawls which often turn into a free-for-all involving team officials and hangers-on and require police intervention.